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We used a bacterial artificial chromosome library to generate and verify sex specific sequence and probes that were important to determine the underlying sex of individuals, a BAC library is constructed by cutting up the dragon DNA, the whole genome, and inserting the fragments into bacterial colonies, one fragment per colony, so you can pull particular dragon sequence out at will.
We believe that after the master sex gene on the sex chromosomes does its work, a cascade of gene regulatory processes (those governing development) is initiated leading to a male or a female hatchling reptile, for the most part, these regulatory processes are buffered in some way from varying temperatures in the nest, but only to a point. At high temperatures, the control of the master sex genes is eroded, and temperature brings in its influence.
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